胶原三维多孔基质常用交联方法

胶原三维多孔基质在组织工程中有广泛应用,但是其降解速率快、机械性能较差,通过交联可以改善其性能。常用的有物理和化学交联方法,物理交联包括热交联、紫外交联、γ射线交联等,化学交联包括使用醛类、碳二亚胺等化学交联剂及京尼平、原花青素等天然交联剂,多种交联方式联用可有效提高交联效果。     

低应变瞬态荷载作用下基桩动力响应解析解

目前广泛采用的基桩低应变检测问题的理论基础是基于平截面假定的一维应力波理论。而实际上,锤击产生的应力波是三维应力波,不仅沿桩身纵向传播,还沿径向传播,对于基桩低应变检测问题平截面假定实际上是不成立的。此时,一维纵波传播问题转换为应力波沿三维柱体传播问题。文章从基桩三维理论出发,忽略桩土径向位移,考虑桩体中应力波沿纵向和径向传播与桩土的相互作用,建立三维条件下桩土系统瞬态振动计算模型,推导得到低应变瞬态荷载作用下基桩三维动力响应的解析解,以期为基桩低应变检测提供新的理论指导。     

A Novel Human TGF-β1 Fusion Protein in Combination with rhBMP-2 Increases Chondro-Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Transforming growth factor-beta (TGF-β) is involved in processes related to the differentiation and maturation of osteoprogenitor cells into osteoblasts. Rat bone marrow (BM) cells were cultured in a collagen-gel containing 0.5% fetal bovine serum (FBS) for 10 days in the presence of rhTGF (recombinant human TGF)-β1-F2, a fusion protein engineered to include a high-affinity collagen-binding decapeptide derived from von Willebrand factor. Subsequently, cells were moderately expanded in medium with 10% FBS for 4 days and treated with a short pulse of rhBMP (recombinant human bone morphogenetic protein)-2 for 4 h. During the last 2 days, dexamethasone and β-glycerophosphate were added to potentiate osteoinduction. Concomitant with an up-regulation of cell proliferation, DNA synthesis levels were determined. Polymerase chain reaction was performed to reveal the possible stemness of these cells. Osteogenic differentiation was evaluated in terms of alkaline phosphatase activity and mineralized matrix formation as well as by mRNA expression of osteogenic marker genes. Moreover, cells were placed inside diffusion chambers and implanted subcutaneously into the backs of adult rats for 4 weeks. Histological study provided evidence of cartilage and bone-like tissue formation. This experimental procedure is capable of selecting cell populations from BM that, in the presence of rhTGF-β1-F2 and rhBMP-2, achieve skeletogenic potential in vitro and in vivo.     

综放巷内充填原位沿空留巷技术

针对综放沿空留巷的技术难点,在分析综放沿空留巷围岩结构力学模型的基础上,提出了巷内充填原位沿空留巷的新技术。此项技术有两个关键方面:一是大断面全煤巷道顶煤完整性的维护;二是大体积长距离充填体的整体稳定性控制。对巷内基本支护为梯形金属支架的原有巷道,提出了实施此项新技术的具体方案。现场工业性试验表明,该项技术的应用是成功而有效的,可在类似条件的综放工作面推广应用。     

带撑式基坑支护结构变形影响因素分析

利用有限元分析软件ANSYS对一典型带撑式基坑工程在开挖过程中的变形进行模拟分析,将模拟计算结果与实测值进行比较,验证该方法的可行性。并进一步对影响基坑变形的几个主要因素：支护结构的墙体刚度、墙体入土深度、支撑刚度、支撑位置、基坑平面尺寸效应及基坑底面以下土体强度等进行系统分析,提出带撑式基坑开挖变形控制的支护设计概念和建议。     

基于三维网络模拟基础的复杂有限块体的自动搜索及其空间几何形态的判定

首先应用随机不连续面三维网络模拟技术建立不连续面分布的概率统计模型。在此基础上通过编程实现对复杂有限块体的自动搜索并确定其空间几何形态，给出其空间定位参数和尺寸。在对块本的有限性进行判断时，提出“有形即是有限”的分析方法。     

Modern Geodetic Methods of Constructing Digital Three-Dimensional Models for the Monitoring of Engineering Structures

A brief review of modern surface geodetic methods used in monitoring projects ranging in size from several tens of meters to several kilometers is presented. Buildings and various engineering structures, as well as slide-prone types of natural entities, pulsating glaciers, etc. are classed among these entities. Use of digital three-dimensional models of observable entities, which are developed from results of laser scanning and/or a digital photogrammetric survey, is proposed.     

夏热冬冷地区三维空间视角下住宅南向窗节能研究

外窗属于外围护结构之一,是建筑的重要组成部分,也是建筑保温中最薄弱的环节。窗的开设与建筑节能密切相关。夏热冬冷地区南向窗应属整套住宅建筑的主要采光源,其窗户面积大小变化对采光率影响较大。以夏热冬冷地区——桂林市南向窗采光良好条件为基础,以"窗墙面积比"和"窗地面积比"作为研究变量,以能耗模拟软件Ecotect模拟作为研究方法,在三维空间视角下进行建筑热环境模拟研究,确定了南向房间节能的"窗墙面积比"与"窗地面积比"取值范围,为今后的夏热冬冷地区——桂林市住宅建筑的节能设计提供一定的参考依据。     

岩坡稳定的三维强度折减法分析

目前，强度折减法基本上用于边坡的二维稳定分析。为研究其在岩石边坡三维稳定分析中的适用性，选择平面滑动和楔形体滑动2个经典算例，运用强度折减法求解其安全系数，并与E．Hoek和J．W.Bray给出的解析解进行对比。计算结果表明，数值解与解析解非常接近，模拟的滑动方向与理论假定也基本一致。算例中，结构面使用实体单元模拟，为此进一步探讨结构面单元厚度和网格密度对计算精度的影响；结构面均为甲面，其厚度为块体高度的1／10～／200，厚度与单元数对安全系数的计算结果影响不大。对特征点位移准则和塑性区贯通准则进行比较分析，认为结构面塑性区贯通收敛准则在理论上与极限平衡理论较为吻合，实际应用上也易于操作．作为收敛准则较为合理。三滑面五面体滑动算例进一步验证该方法的适用性。     

块状结构岩体在一般水压分布模式下的不连续变形分析

提出了三维不连续变形分析方法中地下水压力的模拟方法。并通过钻孔水位观测数据插值拟合地下水位面,建立了作用在任意形状块体单元表面上的一般水压分布模式,利用最小势能原理推导了水压力荷载对三维不连续变形分析方法总刚矩阵的贡献。地下水对滑坡稳定性影响的算例分析表明该方法是有效的。